8:00 am - 9:30 am
 | Cytology Workshop #9
Credit Hours:1.5 CME/CMLE/SAM
Creating a Good Image: Electronic Image Enhancement with Photoshop®
Daniel F. I. Kurtcyz, MD
George G. Birdsong, MD
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Educational Objectives:
1. Discuss the characteristics of quality electronic photomicrographs for presentation or publication
2. Discuss color theory and color balancing as it pertains to photomicrographs
3. Familiarize participants with electronic manipulation of digitized images at a novice level using standard desktop computer tools
4. Explain the sharpening of images and the correction of lighting gradients
5. Explain common image formats used for presentation and publication
In a seminar format, we propose to instruct individuals on the theory and practice of electronic image manipulation using desktop computers and the most popular image editing tool, Adobe Photoshop®. Characteristics of quality electronic photographs will be discussed. We will show how to color balance, brighten and sharpen images. We will also show examples of what should not be acceptable for scientific images. Since almost all presentations are now given by means of computers and electronic projection systems, there is a significant need to teach people how to optimize scanned or captured digitized images for public display.
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8:00 am - 9:30 am
 | Cytology Workshop #10
Credit Hours:1.5 CME/CMLE/SAM
EUS-FNA of the Pancreas: Challenges and Opportunities, A Dialogue Between Endosonographer and Cytopathologist
Nirag C. Jhala, MD, MIAC
Darshana N. Jhala, MD, BMus
Shyam Varadarajulu, MD
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Educational Objectives:
1. Develop a practical approach to diagnosis of solid pancreatic lesions suspected of pancreatic carcinoma
2. Characterize role of multispecialty approach to the diagnosis of mucinous cystic lesions of the pancreas
3. Characterize role of ancillary studies on limited cytology samples
Endoscopic ultrasound guided fine needle aspiration has emerged as a modality of choice in managing patients with pancreatic neoplasms. The success of utilization of this technique depends upon many variables. It is also known that assessment of EUS images by endosonographers and its guided FNA samples from the pancreas remains a significant challenge for cytopathologists with grave patient implications. A development of a sound partnership between the two can provide a solid framework to determine further patient management. This course will utilize a case based approach to demonstrate practical algorithmic approach to arrive at diagnosis of suspected pancreatic carcinomas and differentiate pancreatitis (chronic and autoimmune) from pancreatic carcinoma. This course will also show how to distinguish non ductal pancreatic lesions such as pancreatic endocrine neoplasms from its mimickers. Finally, the course will highlight a partnered approach to a diagnosis of mucinous cystic lesions of the pancreas and opportunities to refine morphologic diagnosis based on available ancillary studies performed on these limited samples.
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8:00 am - 9:30 am
 | Cytology Workshop #11
Credit Hours:1.5 CME/CMLE/SAM
Gynecologic Cytology Jeopardy
Barbara A. Crothers, DO
Kristen E. Natale, MD
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Educational Objectives:
1. Engage audience in collaborative effort to learn new and refresh old criteria for unusual GYN cytology lesions
2. Indicate the appropriate ASCCP follow-up and therapy for abnormal Pap tests
3. Recognize preparation problems and how to deal with them
4. Identify common associations (cellular and clinical findings) in GYN cytology
This interactive educational experience challenges participants to test themselves in multiple categories: GYN preparation problems and how to solve them; cervical cancer screening, follow up and treatment algorithms; misunderstood CLIA regulations; unusual entities found on Pap tests; and common associations in GYN cytology. Most of the questions require a visual clue. The participants form two teams that compete for the most correct answers by sharing information. This format encourages group learning and interaction, and it is fun! Each choice selected is followed with a short summary explaining the correct answer.
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8:00 am - 9:30 am

 | Cytology Workshop #12
Credit Hours:1.5 CME/CMLE/SAM
The Role of Cytology in Molecular Diagnosis, Prognosis and Patient Management in Lung Cancer
Andre L. Moreira, MD
Natasha Rekhtman, MD, PhD
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Educational Objectives:
1. Review current advances in thoracic oncology to bring the participants up-to-date with the concept of target therapy in non-small cell lung cancer (NSCLC)
2. Review the use of immunocytochemistry in the subclassification of NSCLC and evaluation of prognostic markers in lung adenocarcinoma
3. Highlight the importance of critical cytological material for molecular mutation analysis, including EGFR and KRAS mutation, as well as the newly discovered EML4-ALK translocation, and future discoveries
4. Emphasize the important role of cytopathologists and allied professionals in the management of patients with lung cancer, from the specimen acquisition, diagnosis accuracy, and triage for molecular studies
The majority of patients newly diagnosed with lung cancer present with advanced clinical stage, where systemic therapy is the most common therapeutic option. In these cases, cytology or small biopsy specimens are the only mean of tissue diagnosis. Target therapy, the use of specific drugs to address specific molecular alterations discovered in lung cancer, has become one of the most important modalities of systemic therapy. Cytopathologists and allied professionals have become integral to the patient management team by providing accurate diagnosis, crucial prognostic information, and participants in the triage of critical diagnostic material for specific molecular tests. This session will provide the participants with practical information on the utilization of resources to reach adequate and accurate diagnosis as well as procurement and management of tissue for molecular tests.
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8:00 am - 9:30 am
 | Cytology Workshop #13
Credit Hours:1.5 CME/CMLE/SAM
Pap Puzzelers: Challenging and Unusual Cases in GYN Cytopathology
Jamie L. Covell, BS, CT(ASCP)
Karen M. Atkison, MPA, CT(ASCP)
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Educational Objectives:
1. Be able to recognize and discuss differential diagnoses for difficult cases
2. Describe criteria for making morphologic evaluations in unusual cases
3. Discuss the importance of clinical history in the evaluation of Pap tests
The workshop will discuss the cytologic findings and differential diagnoses for a series of challenging and/or unusual Pap test cases. We will evaluate each specimen as a routine screening case seen in the laboratory. Participants, divided into teams, will review images from each case as well as possible differentials and discuss their observations and interpretations. Each team will then electronically vote for their choice among the differential diagnoses under consideration. Cytologic and/or tissue follow-up will confirm or provide the final diagnosis for each case. Interactive participation and lively discussion will be essential. Paps can be fun!
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10:30 am - 12:00 pm
 | Cytology Workshop #14
Credit Hours:1.5 CME/CMLE/SAM
Diagnostic Approach to Urine Cytology
Abdelmonem Elhosseiny, MD
Sandra Giroux, SCT(ASCP), CFIAC
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Educational Objectives:
1. Illustrate the true value of urine cytology in detecting high grade lesions
2. Recognize the pitfalls that could lead to erroneous diagnoses and how to avoid them
3. Explore the role of molecular diagnostics in difficult cases
4. Explain how to perform quality assurance in your own laboratory by correlating urologic findings, cytology and biopsy diagnosis
This workshop is designed to highlight the major role of urinary cytology in correctly diagnosing high grade lesions and how to minimize any false positives by illustrating the various pitfalls that could arise due to viral infections, stones, drugs, radiation, etc. Define the role of molecular testing in urine cytology.
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10:30 am - 12:00 pm
 | Cytology Workshop #15
Credit Hours:1.5 CME/CMLE/SAM
Identification of Infectious Diseases in Cytology Specimens with Histology Correlation and Microbiology Recommendations
Eva M. Wojcik, MD
Sherri Yong, MD
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Educational Objectives:
1. Bridge the gap between the constantly evolving identification of infectious diseases in the clinical microbiology and molecular laboratories and the recognition of infectious disease agents in cytopathology and surgical pathology specimens
2. Help pathologists and cytotechnologists to recognize infectious disease agents in increasingly smaller specimens
3. Make pathologists and cytotechnologists aware of newer molecular techniques and newer culture studies needed for the diagnosis of these organisms and enable them to communicate these information to clinicians and interventional radiologists
4. Update pathologists and cytotechnologists on emerging infections and infectious disease agents that might be encountered as global traveling increases
This didatic teaching course will cover the identification of viral, bacterial, fungal and parasitic infectious disease agents in cytopathology specimens with surgical pathology correlations and recommendations for microbiological studies. This session will address commonly encountered infectious diseases as well as emerging and re-emerging infectious disease agents. Special attention to common infectious agents that might be encountered from global traveling will also be addressed. Participants will be encouraged to share their experiences and diagnostic practices as part of this workshop.
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10:30 am - 12:00 pm

 | Cytology Workshop #16
Credit Hours:1.5 CME/CMLE/SAM
Diagnostic Cytopathology of Serous Effusions
Vinod B. Shidham, MD, FRCPath, FIAC
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Educational Objectives:
1. Approach to cytomorphological evaluation of serous fluid cytology specimens from various benign and malignant conditions
2. Discussion of methods for evaluating immunohistochemistry in cell block sections of serous fluids
3. Emphasize important diagnostic pitfalls
The course will provide a comprehensive overview of the diagnostic framework for evaluating cytology of serous effusions, which are easily obtainable and therefore can be seen in any pathology laboratory. However, paradoxically these specimens present a considerable diagnostic difficulty for practicing pathologists in both community and academic settings. The intended audience includes residents, fellows, and pathologists wishing to refresh and update their knowledge in this field with some revolutionary approaches. Topics covered will be practical issues including specimen collection and processing with emphasis on cytomorphological evaluation with proper application of ancillary tests, especially immunocytochemistry.
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10:30 am - 12:00 pm
 | Cytology Workshop #17
Credit Hours:1.5 CME/CMLE/SAM
Integration of Molecular Studies and Thyroid Cytopathology in the Age of the Bethesda Classification System
N. Paul Ohori, MD
Zubair W. Baloch, MD, PhD
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Educational Objectives:
1. Review the current status of thyroid FNA and the need for ancillary testing
2. Review FNA technique and specimen processing for optimal cytomorphology and molecular testing
3. Review common molecular pathways involved in thyroid neoplasia
4. Discuss the pathologic and clinical significance of molecular alterations in the context of the Thyroid Bethesda Classification System
5. Compare molecular testing to other ancillary tests (e.g. immunochemistry) and discuss the emergence of additional technologies impacting the refinement of cytopathologic diagnoses
Integration of ancillary studies to routine cytopathologic analysis has become a common part of diagnostic practice. Recently, the molecular pathways of common thyroid malignancies have become better understood. In particular, point mutations in BRAF and RAS genes and RET/PTC rearrangements are found in over 70% of papillary thyroid carcinomas. Follicular carcinomas are associated with RAS point mutation and PAX8-PPAR gamma rearrangement in approximately 80% of cases. Incorporation of molecular techniques has the potential of refining the cytologic diagnosis of thyroid FNAs. In this session, we will discuss the current standard of thyroid FNA practice and the need for implementing molecular testing. Specific emphasis will be placed on optimal techniques for obtaining samples for molecular studies and the interpretation of molecular results in the context of the Thyroid Bethesda Classification diagnoses. The discussion will include how molecular testing can improve the pre-surgical cytologic practice so that the cytopathologist can better guide the clinicians in managing the patients.
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10:30 am - 12:00 pm

 | Cytology Workshop #18
Credit Hours:1.5 CME/CMLE/SAM
Optimizing FNA Technique with an Introduction to US Guidance - A Practical Hands-on Workshop
Ronald Balassanian, MD
Britt-Marie E. Ljung, MD
Elham Khanafshar, MD
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Educational Objectives:
1. Learn effective FNA techniques, including needle placement, sampling different types of lesions (reactive lymph node versus sclerotic carcinoma versus cystic lesion)
2. Learn effective smearing technique to get oprimal smears every time
3. Learn effective ways to divide samples to maximize the yield from the FNA, including dealing with bloody or dilute samples, and obtaining material for ancillary studies (flow cytometry, molecular testing and cell blocks)
4. Learn techniques to deal with emotional, difficult or confrontational patients
5. Introduction to ultrasound guidance for needle placement
This interactive hands-on workshop is geared to cytology fellows, residents and practicing cytologists, including cytotechnologists involved with FNA procurement. The workshop is well suited for individuals with limited opportunities for performing FNA but will also benefit those working in busy or active FNA practices who would like to sharpen their skills and learn some new techniques to refine and optimize their samples. Prior to the workshop, participants will be asked to review Dr. Ljung's instructional video on FNA available on-line or on DVD. The workshop will be divided into 4 components. First there will be a short lecture summarizing the main points of Dr. Ljung's video, followed by a live demonstration of the biopsy techniques using cow liver and other props as needed. Second, we will then divide the participants into small groups of 3 or 4 with an instructor. Each participant will have an opportunity to practice the techniques demonstrated, with the instructor present to offer advice and correction as needed. Third, we will re-convene the class to for a group demonstration and reinforce the techniques learned. We will also introduce ultrasound guidance for needle placement using an US machine and phantoms. The fourth session will consist of discussing techniques for dealing with difficult, emotional or combative patients with short role-play scenarios using volunteer participants as cytologists and instructors as difficult patients.
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